negative control plasmids oe-nc (Shanghai GenePharma)
90
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Shanghai GenePharma
negative control plasmids oe-nc
Negative Control Plasmids Oe Nc, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+(nc)+plasmids/negative+control+plasmid+sh+nc/pmc11847424-101-5-12
Average 90 stars, based on 1 article reviews
Negative Control Plasmids Oe Nc, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+(nc)+plasmids/negative+control+plasmid+sh+nc/pmc11847424-101-5-12
Average 90 stars, based on 1 article reviews
negative control plasmids oe-nc - by Bioz Stars,
2026-09
90/100 stars
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shRNA:Article Title: SIRT3 mitigates neuroinflammation and mitochondrial damage post-hypoxic-ischemic brain injury. Article Snippet: Objective: We aimed to explore the role of SIRT3 in ameliorating neuroinflammation caused by hypoxia-ischemia Article Title: Hydrogen mitigates brain injury by prompting NEDD4-CX43- mediated mitophagy in traumatic brain injury. Article Snippet: Background: Hydrogen (H2) has emerged as a potential therapeutic intervention for traumatic brain injury (TBI).. However, the precise mechanism underlying H2's neuroprotective effects in TBI remain incompletely understood.. Methods: TBI mouse model was induced using the controlled cortical impact (CCI) method, and a cell model was established by exposing astrocytes to lipopolysaccharide (LPS). Article Title: SIRT1 regulates cigarette smoke extract‑induced alveolar macrophage polarization and inflammation by inhibiting the TRAF6/NLRP3 signaling pathway Article Snippet: The overexpression plasmids of SIRT1 [Ov-SIRT1; vector, pEX-3(pGCMV/MCS/Neo)], TRAF6 [(Ov-TRAF6; vector, pEX-3(pGCMV/MCS/Neo)] and negative control (NC) plasmid pEX-3(pGCMV/MCS/Neo) were purchased from Article Title: IGFBP5 promotes EndoMT and renal fibrosis through H3K18 lactylation in diabetic nephropathy. Article Snippet: Short hairpin RNA (shRNA) against IGFBP5 (sh-IGFBP5) and negative control (sh-NC) plasmids were designed (GenePharma, Shanghai, China) to specifically knock down IGFBP5 expression in GEnCs. Article Title: Diosgenin attenuates nonalcoholic fatty liver disease through mTOR-mediated inhibition of lipid accumulation and inflammation. Article Snippet: Excessive hepatic lipid accumulation and inflammatory injury are significant pathological manifestations of nonalcoholic fatty liver disease (NAFLD).. Our previous research discovered that diosgenin, a natural steroidal saponin derived from Chinese herbs, can reduce hepatic lipid accumulation and steatosis; however, the exact mechanism remains unclear.. This study aimed to investigate the protective mechanisms of diosgenin against NAFLD. Article Title: Apigenin alleviates Sjögren's syndrome-induced salivary gland epithelial cell ferroptosis via ERα signaling-mediated regulation of the ATF3/SLC7A1l axis. Article Snippet: Background: In Sjögren’s syndrome (SS)—an autoimmune disease characterized by dry mouth and eyes—salivary gland epithelial cells (SGECs) undergo ferroptosis, which disrupts their integrity and impairs saliva secretion.. Apigenin, a phytoestrogen, is known to activate estrogen signalling and alleviate xerostomia in ovariectomized mice; however, its effect on SGEC survival and function in SS remains unclear.. We hypothesized that apigenin alleviates SS symptoms and progression by inhibiting ferroptosis in SGECs and aimed to elucidate the underlying mechanism. Article Title: c-Myc-dependent LAMP3 regulates the proliferation, metastasis and metabolic reprogramming of tongue squamous cell carcinoma. Article Snippet: Small hairpin RNA specifically targeting LAMP3 (sh-LAMP3), shRNA negative control (sh-NC); c-Myc overexpression plasmid pcDNA3.1-c-Myc (Oec-Myc), and empty pcDNA3.1 plasmid (Oe-NC) were obtained from GenePharma (Shanghai, China). Article Title: Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1 Article Snippet: EPB41L4A-AS1 overexpression plasmids (oe-EPB41L4A-AS1) and negative control plasmids (oe-NC) were obtained from GenePharma. Negative Control:Article Title: SIRT3 mitigates neuroinflammation and mitochondrial damage post-hypoxic-ischemic brain injury. Article Snippet: Objective: We aimed to explore the role of SIRT3 in ameliorating neuroinflammation caused by hypoxia-ischemia Article Title: Hydrogen mitigates brain injury by prompting NEDD4-CX43- mediated mitophagy in traumatic brain injury. Article Snippet: Background: Hydrogen (H2) has emerged as a potential therapeutic intervention for traumatic brain injury (TBI).. However, the precise mechanism underlying H2's neuroprotective effects in TBI remain incompletely understood.. Methods: TBI mouse model was induced using the controlled cortical impact (CCI) method, and a cell model was established by exposing astrocytes to lipopolysaccharide (LPS). Article Title: SIRT1 regulates cigarette smoke extract‑induced alveolar macrophage polarization and inflammation by inhibiting the TRAF6/NLRP3 signaling pathway Article Snippet: The overexpression plasmids of SIRT1 [Ov-SIRT1; vector, pEX-3(pGCMV/MCS/Neo)], TRAF6 [(Ov-TRAF6; vector, pEX-3(pGCMV/MCS/Neo)] and negative control (NC) plasmid pEX-3(pGCMV/MCS/Neo) were purchased from Article Title: IGFBP5 promotes EndoMT and renal fibrosis through H3K18 lactylation in diabetic nephropathy. Article Snippet: Short hairpin RNA (shRNA) against IGFBP5 (sh-IGFBP5) and negative control (sh-NC) plasmids were designed (GenePharma, Shanghai, China) to specifically knock down IGFBP5 expression in GEnCs. Article Title: Diosgenin attenuates nonalcoholic fatty liver disease through mTOR-mediated inhibition of lipid accumulation and inflammation. Article Snippet: Excessive hepatic lipid accumulation and inflammatory injury are significant pathological manifestations of nonalcoholic fatty liver disease (NAFLD).. Our previous research discovered that diosgenin, a natural steroidal saponin derived from Chinese herbs, can reduce hepatic lipid accumulation and steatosis; however, the exact mechanism remains unclear.. This study aimed to investigate the protective mechanisms of diosgenin against NAFLD. Article Title: Apigenin alleviates Sjögren's syndrome-induced salivary gland epithelial cell ferroptosis via ERα signaling-mediated regulation of the ATF3/SLC7A1l axis. Article Snippet: Background: In Sjögren’s syndrome (SS)—an autoimmune disease characterized by dry mouth and eyes—salivary gland epithelial cells (SGECs) undergo ferroptosis, which disrupts their integrity and impairs saliva secretion.. Apigenin, a phytoestrogen, is known to activate estrogen signalling and alleviate xerostomia in ovariectomized mice; however, its effect on SGEC survival and function in SS remains unclear.. We hypothesized that apigenin alleviates SS symptoms and progression by inhibiting ferroptosis in SGECs and aimed to elucidate the underlying mechanism. Article Title: c-Myc-dependent LAMP3 regulates the proliferation, metastasis and metabolic reprogramming of tongue squamous cell carcinoma. Article Snippet: Small hairpin RNA specifically targeting LAMP3 (sh-LAMP3), shRNA negative control (sh-NC); c-Myc overexpression plasmid pcDNA3.1-c-Myc (Oec-Myc), and empty pcDNA3.1 plasmid (Oe-NC) were obtained from GenePharma (Shanghai, China). Article Title: Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1 Article Snippet: EPB41L4A-AS1 overexpression plasmids (oe-EPB41L4A-AS1) and negative control plasmids (oe-NC) were obtained from GenePharma. Over Expression:Article Title: SIRT3 mitigates neuroinflammation and mitochondrial damage post-hypoxic-ischemic brain injury. Article Snippet: Objective: We aimed to explore the role of SIRT3 in ameliorating neuroinflammation caused by hypoxia-ischemia Article Title: Hydrogen mitigates brain injury by prompting NEDD4-CX43- mediated mitophagy in traumatic brain injury. Article Snippet: Background: Hydrogen (H2) has emerged as a potential therapeutic intervention for traumatic brain injury (TBI).. However, the precise mechanism underlying H2's neuroprotective effects in TBI remain incompletely understood.. Methods: TBI mouse model was induced using the controlled cortical impact (CCI) method, and a cell model was established by exposing astrocytes to lipopolysaccharide (LPS). Article Title: SIRT1 regulates cigarette smoke extract‑induced alveolar macrophage polarization and inflammation by inhibiting the TRAF6/NLRP3 signaling pathway Article Snippet: The overexpression plasmids of SIRT1 [Ov-SIRT1; vector, pEX-3(pGCMV/MCS/Neo)], TRAF6 [(Ov-TRAF6; vector, pEX-3(pGCMV/MCS/Neo)] and negative control (NC) plasmid pEX-3(pGCMV/MCS/Neo) were purchased from Article Title: IGFBP5 promotes EndoMT and renal fibrosis through H3K18 lactylation in diabetic nephropathy. Article Snippet: Short hairpin RNA (shRNA) against IGFBP5 (sh-IGFBP5) and negative control (sh-NC) plasmids were designed (GenePharma, Shanghai, China) to specifically knock down IGFBP5 expression in GEnCs. Article Title: Diosgenin attenuates nonalcoholic fatty liver disease through mTOR-mediated inhibition of lipid accumulation and inflammation. Article Snippet: Excessive hepatic lipid accumulation and inflammatory injury are significant pathological manifestations of nonalcoholic fatty liver disease (NAFLD).. Our previous research discovered that diosgenin, a natural steroidal saponin derived from Chinese herbs, can reduce hepatic lipid accumulation and steatosis; however, the exact mechanism remains unclear.. This study aimed to investigate the protective mechanisms of diosgenin against NAFLD. Article Title: Apigenin alleviates Sjögren's syndrome-induced salivary gland epithelial cell ferroptosis via ERα signaling-mediated regulation of the ATF3/SLC7A1l axis. Article Snippet: Background: In Sjögren’s syndrome (SS)—an autoimmune disease characterized by dry mouth and eyes—salivary gland epithelial cells (SGECs) undergo ferroptosis, which disrupts their integrity and impairs saliva secretion.. Apigenin, a phytoestrogen, is known to activate estrogen signalling and alleviate xerostomia in ovariectomized mice; however, its effect on SGEC survival and function in SS remains unclear.. We hypothesized that apigenin alleviates SS symptoms and progression by inhibiting ferroptosis in SGECs and aimed to elucidate the underlying mechanism. Article Title: c-Myc-dependent LAMP3 regulates the proliferation, metastasis and metabolic reprogramming of tongue squamous cell carcinoma. Article Snippet: Small hairpin RNA specifically targeting LAMP3 (sh-LAMP3), shRNA negative control (sh-NC); c-Myc overexpression plasmid pcDNA3.1-c-Myc (Oec-Myc), and empty pcDNA3.1 plasmid (Oe-NC) were obtained from GenePharma (Shanghai, China). Article Title: Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1 Article Snippet: EPB41L4A-AS1 overexpression plasmids (oe-EPB41L4A-AS1) and negative control plasmids (oe-NC) were obtained from GenePharma. Plasmid Preparation:Article Title: SIRT3 mitigates neuroinflammation and mitochondrial damage post-hypoxic-ischemic brain injury. Article Snippet: Objective: We aimed to explore the role of SIRT3 in ameliorating neuroinflammation caused by hypoxia-ischemia Article Title: Hydrogen mitigates brain injury by prompting NEDD4-CX43- mediated mitophagy in traumatic brain injury. Article Snippet: Background: Hydrogen (H2) has emerged as a potential therapeutic intervention for traumatic brain injury (TBI).. However, the precise mechanism underlying H2's neuroprotective effects in TBI remain incompletely understood.. Methods: TBI mouse model was induced using the controlled cortical impact (CCI) method, and a cell model was established by exposing astrocytes to lipopolysaccharide (LPS). Article Title: SIRT1 regulates cigarette smoke extract‑induced alveolar macrophage polarization and inflammation by inhibiting the TRAF6/NLRP3 signaling pathway Article Snippet: The overexpression plasmids of SIRT1 [Ov-SIRT1; vector, pEX-3(pGCMV/MCS/Neo)], TRAF6 [(Ov-TRAF6; vector, pEX-3(pGCMV/MCS/Neo)] and negative control (NC) plasmid pEX-3(pGCMV/MCS/Neo) were purchased from Article Title: IGFBP5 promotes EndoMT and renal fibrosis through H3K18 lactylation in diabetic nephropathy. Article Snippet: Short hairpin RNA (shRNA) against IGFBP5 (sh-IGFBP5) and negative control (sh-NC) plasmids were designed (GenePharma, Shanghai, China) to specifically knock down IGFBP5 expression in GEnCs. Article Title: Diosgenin attenuates nonalcoholic fatty liver disease through mTOR-mediated inhibition of lipid accumulation and inflammation. Article Snippet: Excessive hepatic lipid accumulation and inflammatory injury are significant pathological manifestations of nonalcoholic fatty liver disease (NAFLD).. Our previous research discovered that diosgenin, a natural steroidal saponin derived from Chinese herbs, can reduce hepatic lipid accumulation and steatosis; however, the exact mechanism remains unclear.. This study aimed to investigate the protective mechanisms of diosgenin against NAFLD. Article Title: Apigenin alleviates Sjögren's syndrome-induced salivary gland epithelial cell ferroptosis via ERα signaling-mediated regulation of the ATF3/SLC7A1l axis. Article Snippet: Background: In Sjögren’s syndrome (SS)—an autoimmune disease characterized by dry mouth and eyes—salivary gland epithelial cells (SGECs) undergo ferroptosis, which disrupts their integrity and impairs saliva secretion.. Apigenin, a phytoestrogen, is known to activate estrogen signalling and alleviate xerostomia in ovariectomized mice; however, its effect on SGEC survival and function in SS remains unclear.. We hypothesized that apigenin alleviates SS symptoms and progression by inhibiting ferroptosis in SGECs and aimed to elucidate the underlying mechanism. Article Title: c-Myc-dependent LAMP3 regulates the proliferation, metastasis and metabolic reprogramming of tongue squamous cell carcinoma. Article Snippet: Small hairpin RNA specifically targeting LAMP3 (sh-LAMP3), shRNA negative control (sh-NC); c-Myc overexpression plasmid pcDNA3.1-c-Myc (Oec-Myc), and empty pcDNA3.1 plasmid (Oe-NC) were obtained from GenePharma (Shanghai, China). Article Title: Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1 Article Snippet: EPB41L4A-AS1 overexpression plasmids (oe-EPB41L4A-AS1) and negative control plasmids (oe-NC) were obtained from GenePharma. Knockdown:Article Title: SIRT3 mitigates neuroinflammation and mitochondrial damage post-hypoxic-ischemic brain injury. Article Snippet: Objective: We aimed to explore the role of SIRT3 in ameliorating neuroinflammation caused by hypoxia-ischemia Article Title: Hydrogen mitigates brain injury by prompting NEDD4-CX43- mediated mitophagy in traumatic brain injury. Article Snippet: Background: Hydrogen (H2) has emerged as a potential therapeutic intervention for traumatic brain injury (TBI).. However, the precise mechanism underlying H2's neuroprotective effects in TBI remain incompletely understood.. Methods: TBI mouse model was induced using the controlled cortical impact (CCI) method, and a cell model was established by exposing astrocytes to lipopolysaccharide (LPS). Article Title: SIRT1 regulates cigarette smoke extract‑induced alveolar macrophage polarization and inflammation by inhibiting the TRAF6/NLRP3 signaling pathway Article Snippet: The overexpression plasmids of SIRT1 [Ov-SIRT1; vector, pEX-3(pGCMV/MCS/Neo)], TRAF6 [(Ov-TRAF6; vector, pEX-3(pGCMV/MCS/Neo)] and negative control (NC) plasmid pEX-3(pGCMV/MCS/Neo) were purchased from Article Title: IGFBP5 promotes EndoMT and renal fibrosis through H3K18 lactylation in diabetic nephropathy. Article Snippet: Short hairpin RNA (shRNA) against IGFBP5 (sh-IGFBP5) and negative control (sh-NC) plasmids were designed (GenePharma, Shanghai, China) to specifically knock down IGFBP5 expression in GEnCs. Article Title: Diosgenin attenuates nonalcoholic fatty liver disease through mTOR-mediated inhibition of lipid accumulation and inflammation. Article Snippet: Excessive hepatic lipid accumulation and inflammatory injury are significant pathological manifestations of nonalcoholic fatty liver disease (NAFLD).. Our previous research discovered that diosgenin, a natural steroidal saponin derived from Chinese herbs, can reduce hepatic lipid accumulation and steatosis; however, the exact mechanism remains unclear.. This study aimed to investigate the protective mechanisms of diosgenin against NAFLD. Article Title: Apigenin alleviates Sjögren's syndrome-induced salivary gland epithelial cell ferroptosis via ERα signaling-mediated regulation of the ATF3/SLC7A1l axis. Article Snippet: Background: In Sjögren’s syndrome (SS)—an autoimmune disease characterized by dry mouth and eyes—salivary gland epithelial cells (SGECs) undergo ferroptosis, which disrupts their integrity and impairs saliva secretion.. Apigenin, a phytoestrogen, is known to activate estrogen signalling and alleviate xerostomia in ovariectomized mice; however, its effect on SGEC survival and function in SS remains unclear.. We hypothesized that apigenin alleviates SS symptoms and progression by inhibiting ferroptosis in SGECs and aimed to elucidate the underlying mechanism. Article Title: c-Myc-dependent LAMP3 regulates the proliferation, metastasis and metabolic reprogramming of tongue squamous cell carcinoma. Article Snippet: Small hairpin RNA specifically targeting LAMP3 (sh-LAMP3), shRNA negative control (sh-NC); c-Myc overexpression plasmid pcDNA3.1-c-Myc (Oec-Myc), and empty pcDNA3.1 plasmid (Oe-NC) were obtained from GenePharma (Shanghai, China). Article Title: Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1 Article Snippet: EPB41L4A-AS1 overexpression plasmids (oe-EPB41L4A-AS1) and negative control plasmids (oe-NC) were obtained from GenePharma. Expressing:Article Title: SIRT3 mitigates neuroinflammation and mitochondrial damage post-hypoxic-ischemic brain injury. Article Snippet: Objective: We aimed to explore the role of SIRT3 in ameliorating neuroinflammation caused by hypoxia-ischemia Article Title: Hydrogen mitigates brain injury by prompting NEDD4-CX43- mediated mitophagy in traumatic brain injury. Article Snippet: Background: Hydrogen (H2) has emerged as a potential therapeutic intervention for traumatic brain injury (TBI).. However, the precise mechanism underlying H2's neuroprotective effects in TBI remain incompletely understood.. Methods: TBI mouse model was induced using the controlled cortical impact (CCI) method, and a cell model was established by exposing astrocytes to lipopolysaccharide (LPS). Article Title: SIRT1 regulates cigarette smoke extract‑induced alveolar macrophage polarization and inflammation by inhibiting the TRAF6/NLRP3 signaling pathway Article Snippet: The overexpression plasmids of SIRT1 [Ov-SIRT1; vector, pEX-3(pGCMV/MCS/Neo)], TRAF6 [(Ov-TRAF6; vector, pEX-3(pGCMV/MCS/Neo)] and negative control (NC) plasmid pEX-3(pGCMV/MCS/Neo) were purchased from Article Title: IGFBP5 promotes EndoMT and renal fibrosis through H3K18 lactylation in diabetic nephropathy. Article Snippet: Short hairpin RNA (shRNA) against IGFBP5 (sh-IGFBP5) and negative control (sh-NC) plasmids were designed (GenePharma, Shanghai, China) to specifically knock down IGFBP5 expression in GEnCs. Article Title: Diosgenin attenuates nonalcoholic fatty liver disease through mTOR-mediated inhibition of lipid accumulation and inflammation. Article Snippet: Excessive hepatic lipid accumulation and inflammatory injury are significant pathological manifestations of nonalcoholic fatty liver disease (NAFLD).. Our previous research discovered that diosgenin, a natural steroidal saponin derived from Chinese herbs, can reduce hepatic lipid accumulation and steatosis; however, the exact mechanism remains unclear.. This study aimed to investigate the protective mechanisms of diosgenin against NAFLD. Article Title: Apigenin alleviates Sjögren's syndrome-induced salivary gland epithelial cell ferroptosis via ERα signaling-mediated regulation of the ATF3/SLC7A1l axis. Article Snippet: Background: In Sjögren’s syndrome (SS)—an autoimmune disease characterized by dry mouth and eyes—salivary gland epithelial cells (SGECs) undergo ferroptosis, which disrupts their integrity and impairs saliva secretion.. Apigenin, a phytoestrogen, is known to activate estrogen signalling and alleviate xerostomia in ovariectomized mice; however, its effect on SGEC survival and function in SS remains unclear.. We hypothesized that apigenin alleviates SS symptoms and progression by inhibiting ferroptosis in SGECs and aimed to elucidate the underlying mechanism. Article Title: c-Myc-dependent LAMP3 regulates the proliferation, metastasis and metabolic reprogramming of tongue squamous cell carcinoma. Article Snippet: Small hairpin RNA specifically targeting LAMP3 (sh-LAMP3), shRNA negative control (sh-NC); c-Myc overexpression plasmid pcDNA3.1-c-Myc (Oec-Myc), and empty pcDNA3.1 plasmid (Oe-NC) were obtained from GenePharma (Shanghai, China). Article Title: Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1 Article Snippet: EPB41L4A-AS1 overexpression plasmids (oe-EPB41L4A-AS1) and negative control plasmids (oe-NC) were obtained from GenePharma. Construct:Article Title: SIRT3 mitigates neuroinflammation and mitochondrial damage post-hypoxic-ischemic brain injury. Article Snippet: Objective: We aimed to explore the role of SIRT3 in ameliorating neuroinflammation caused by hypoxia-ischemia Article Title: Hydrogen mitigates brain injury by prompting NEDD4-CX43- mediated mitophagy in traumatic brain injury. Article Snippet: Background: Hydrogen (H2) has emerged as a potential therapeutic intervention for traumatic brain injury (TBI).. However, the precise mechanism underlying H2's neuroprotective effects in TBI remain incompletely understood.. Methods: TBI mouse model was induced using the controlled cortical impact (CCI) method, and a cell model was established by exposing astrocytes to lipopolysaccharide (LPS). Article Title: SIRT1 regulates cigarette smoke extract‑induced alveolar macrophage polarization and inflammation by inhibiting the TRAF6/NLRP3 signaling pathway Article Snippet: The overexpression plasmids of SIRT1 [Ov-SIRT1; vector, pEX-3(pGCMV/MCS/Neo)], TRAF6 [(Ov-TRAF6; vector, pEX-3(pGCMV/MCS/Neo)] and negative control (NC) plasmid pEX-3(pGCMV/MCS/Neo) were purchased from Article Title: IGFBP5 promotes EndoMT and renal fibrosis through H3K18 lactylation in diabetic nephropathy. Article Snippet: Short hairpin RNA (shRNA) against IGFBP5 (sh-IGFBP5) and negative control (sh-NC) plasmids were designed (GenePharma, Shanghai, China) to specifically knock down IGFBP5 expression in GEnCs. Article Title: Diosgenin attenuates nonalcoholic fatty liver disease through mTOR-mediated inhibition of lipid accumulation and inflammation. Article Snippet: Excessive hepatic lipid accumulation and inflammatory injury are significant pathological manifestations of nonalcoholic fatty liver disease (NAFLD).. Our previous research discovered that diosgenin, a natural steroidal saponin derived from Chinese herbs, can reduce hepatic lipid accumulation and steatosis; however, the exact mechanism remains unclear.. This study aimed to investigate the protective mechanisms of diosgenin against NAFLD. Article Title: Apigenin alleviates Sjögren's syndrome-induced salivary gland epithelial cell ferroptosis via ERα signaling-mediated regulation of the ATF3/SLC7A1l axis. Article Snippet: Background: In Sjögren’s syndrome (SS)—an autoimmune disease characterized by dry mouth and eyes—salivary gland epithelial cells (SGECs) undergo ferroptosis, which disrupts their integrity and impairs saliva secretion.. Apigenin, a phytoestrogen, is known to activate estrogen signalling and alleviate xerostomia in ovariectomized mice; however, its effect on SGEC survival and function in SS remains unclear.. We hypothesized that apigenin alleviates SS symptoms and progression by inhibiting ferroptosis in SGECs and aimed to elucidate the underlying mechanism. Article Title: c-Myc-dependent LAMP3 regulates the proliferation, metastasis and metabolic reprogramming of tongue squamous cell carcinoma. Article Snippet: Small hairpin RNA specifically targeting LAMP3 (sh-LAMP3), shRNA negative control (sh-NC); c-Myc overexpression plasmid pcDNA3.1-c-Myc (Oec-Myc), and empty pcDNA3.1 plasmid (Oe-NC) were obtained from GenePharma (Shanghai, China). Article Title: Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1 Article Snippet: EPB41L4A-AS1 overexpression plasmids (oe-EPB41L4A-AS1) and negative control plasmids (oe-NC) were obtained from GenePharma. Small Interfering RNA:Article Title: SIRT3 mitigates neuroinflammation and mitochondrial damage post-hypoxic-ischemic brain injury. Article Snippet: Objective: We aimed to explore the role of SIRT3 in ameliorating neuroinflammation caused by hypoxia-ischemia Article Title: Hydrogen mitigates brain injury by prompting NEDD4-CX43- mediated mitophagy in traumatic brain injury. Article Snippet: Background: Hydrogen (H2) has emerged as a potential therapeutic intervention for traumatic brain injury (TBI).. However, the precise mechanism underlying H2's neuroprotective effects in TBI remain incompletely understood.. Methods: TBI mouse model was induced using the controlled cortical impact (CCI) method, and a cell model was established by exposing astrocytes to lipopolysaccharide (LPS). Article Title: SIRT1 regulates cigarette smoke extract‑induced alveolar macrophage polarization and inflammation by inhibiting the TRAF6/NLRP3 signaling pathway Article Snippet: The overexpression plasmids of SIRT1 [Ov-SIRT1; vector, pEX-3(pGCMV/MCS/Neo)], TRAF6 [(Ov-TRAF6; vector, pEX-3(pGCMV/MCS/Neo)] and negative control (NC) plasmid pEX-3(pGCMV/MCS/Neo) were purchased from Article Title: IGFBP5 promotes EndoMT and renal fibrosis through H3K18 lactylation in diabetic nephropathy. Article Snippet: Short hairpin RNA (shRNA) against IGFBP5 (sh-IGFBP5) and negative control (sh-NC) plasmids were designed (GenePharma, Shanghai, China) to specifically knock down IGFBP5 expression in GEnCs. Article Title: Diosgenin attenuates nonalcoholic fatty liver disease through mTOR-mediated inhibition of lipid accumulation and inflammation. Article Snippet: Excessive hepatic lipid accumulation and inflammatory injury are significant pathological manifestations of nonalcoholic fatty liver disease (NAFLD).. Our previous research discovered that diosgenin, a natural steroidal saponin derived from Chinese herbs, can reduce hepatic lipid accumulation and steatosis; however, the exact mechanism remains unclear.. This study aimed to investigate the protective mechanisms of diosgenin against NAFLD. Article Title: Apigenin alleviates Sjögren's syndrome-induced salivary gland epithelial cell ferroptosis via ERα signaling-mediated regulation of the ATF3/SLC7A1l axis. Article Snippet: Background: In Sjögren’s syndrome (SS)—an autoimmune disease characterized by dry mouth and eyes—salivary gland epithelial cells (SGECs) undergo ferroptosis, which disrupts their integrity and impairs saliva secretion.. Apigenin, a phytoestrogen, is known to activate estrogen signalling and alleviate xerostomia in ovariectomized mice; however, its effect on SGEC survival and function in SS remains unclear.. We hypothesized that apigenin alleviates SS symptoms and progression by inhibiting ferroptosis in SGECs and aimed to elucidate the underlying mechanism. Article Title: c-Myc-dependent LAMP3 regulates the proliferation, metastasis and metabolic reprogramming of tongue squamous cell carcinoma. Article Snippet: Small hairpin RNA specifically targeting LAMP3 (sh-LAMP3), shRNA negative control (sh-NC); c-Myc overexpression plasmid pcDNA3.1-c-Myc (Oec-Myc), and empty pcDNA3.1 plasmid (Oe-NC) were obtained from GenePharma (Shanghai, China). Article Title: Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1 Article Snippet: EPB41L4A-AS1 overexpression plasmids (oe-EPB41L4A-AS1) and negative control plasmids (oe-NC) were obtained from GenePharma. Synthesized:Article Title: SIRT3 mitigates neuroinflammation and mitochondrial damage post-hypoxic-ischemic brain injury. Article Snippet: Objective: We aimed to explore the role of SIRT3 in ameliorating neuroinflammation caused by hypoxia-ischemia Article Title: Hydrogen mitigates brain injury by prompting NEDD4-CX43- mediated mitophagy in traumatic brain injury. Article Snippet: Background: Hydrogen (H2) has emerged as a potential therapeutic intervention for traumatic brain injury (TBI).. However, the precise mechanism underlying H2's neuroprotective effects in TBI remain incompletely understood.. Methods: TBI mouse model was induced using the controlled cortical impact (CCI) method, and a cell model was established by exposing astrocytes to lipopolysaccharide (LPS). Article Title: SIRT1 regulates cigarette smoke extract‑induced alveolar macrophage polarization and inflammation by inhibiting the TRAF6/NLRP3 signaling pathway Article Snippet: The overexpression plasmids of SIRT1 [Ov-SIRT1; vector, pEX-3(pGCMV/MCS/Neo)], TRAF6 [(Ov-TRAF6; vector, pEX-3(pGCMV/MCS/Neo)] and negative control (NC) plasmid pEX-3(pGCMV/MCS/Neo) were purchased from Article Title: IGFBP5 promotes EndoMT and renal fibrosis through H3K18 lactylation in diabetic nephropathy. Article Snippet: Short hairpin RNA (shRNA) against IGFBP5 (sh-IGFBP5) and negative control (sh-NC) plasmids were designed (GenePharma, Shanghai, China) to specifically knock down IGFBP5 expression in GEnCs. Article Title: Diosgenin attenuates nonalcoholic fatty liver disease through mTOR-mediated inhibition of lipid accumulation and inflammation. Article Snippet: Excessive hepatic lipid accumulation and inflammatory injury are significant pathological manifestations of nonalcoholic fatty liver disease (NAFLD).. Our previous research discovered that diosgenin, a natural steroidal saponin derived from Chinese herbs, can reduce hepatic lipid accumulation and steatosis; however, the exact mechanism remains unclear.. This study aimed to investigate the protective mechanisms of diosgenin against NAFLD. Article Title: Apigenin alleviates Sjögren's syndrome-induced salivary gland epithelial cell ferroptosis via ERα signaling-mediated regulation of the ATF3/SLC7A1l axis. Article Snippet: Background: In Sjögren’s syndrome (SS)—an autoimmune disease characterized by dry mouth and eyes—salivary gland epithelial cells (SGECs) undergo ferroptosis, which disrupts their integrity and impairs saliva secretion.. Apigenin, a phytoestrogen, is known to activate estrogen signalling and alleviate xerostomia in ovariectomized mice; however, its effect on SGEC survival and function in SS remains unclear.. We hypothesized that apigenin alleviates SS symptoms and progression by inhibiting ferroptosis in SGECs and aimed to elucidate the underlying mechanism. Article Title: c-Myc-dependent LAMP3 regulates the proliferation, metastasis and metabolic reprogramming of tongue squamous cell carcinoma. Article Snippet: Small hairpin RNA specifically targeting LAMP3 (sh-LAMP3), shRNA negative control (sh-NC); c-Myc overexpression plasmid pcDNA3.1-c-Myc (Oec-Myc), and empty pcDNA3.1 plasmid (Oe-NC) were obtained from GenePharma (Shanghai, China). Article Title: Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1 Article Snippet: EPB41L4A-AS1 overexpression plasmids (oe-EPB41L4A-AS1) and negative control plasmids (oe-NC) were obtained from GenePharma. |